Tag Archives: cloning

Friday 05/04/12

Posted on May 4, 2012 by admin

12:00 P – 9:30 P 8:30-12P volleyball practice update probe & plasmid library update antibody labels collect virgins set up shRNA crosses x Pc shRNA2 and Psc shRNA 1 in collection cages PCR for in vitro transcription rxn to make … Continue reading →

Posted in Fly Work, Summaries | Tagged , , , | Comments Off on Friday 05/04/12

Tuesday 05/01/12

Posted on May 1, 2012 by admin

11:00 A – 6:45P, 8:00P -12:00A flip fly collection cages, 11A Miniprep cultured colonies: 8E, 7E, 2E, AbdB2 and Fab8 (2 colonies each).  Send for sequencing. Rearrange ultratome room, suspend on air-table, clean room. Flip collection cages, 3:15 A Analyzing … Continue reading →

Posted in Summaries | Tagged , , , | Comments Off on Tuesday 05/01/12

Monday 04/30/12

Posted on April 30, 2012 by admin

9:45 A – 8:15P, Steven group meeting presentation sequencing results: AbdB-1A: sense strand.  Use SP6 for antisense probe AbdB-1B: sense strand.  Use SP6 AbdB-3A: sense strand. poor quality read AbdB-3B: antisense strand. Use T7 Dfd-2A: antisense strand: T7 Dfd-2B: antisense … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Monday 04/30/12

Friday 04/27/12

Posted on April 27, 2012 by admin

10:20A – 5:10P, 9:00P – 11:00P Etch hb-y(i), Bcd sample STORM hb-y(i), Bcd sample remember to turn 642 laser power back up! miniprep colonies: AbdB-1A,B AbdB-3A,B, Dfd-2A,B, 5E-A,B.  all over 100 ng/uL. send 500 ng for sequencing to GeneWiz. move ligation … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Friday 04/27/12

Thursday 05/26/12

Posted on April 26, 2012 by admin

10:00 A – 11:45 P Check colonies from Bx-C round 1 cloning: all plates look good, no lawns. Gel-extract linearized Taf, Tub Gel-extract newly cloned Bx-C probes Antibody synthesis: antibodies 61-69 Flip collection cages: 10:15 A Washing out primary antibody … Continue reading →

Posted in probe and plasmid building, Summaries | Tagged , , , | Comments Off on Thursday 05/26/12

Friday 04/13/12

Posted on April 13, 2012 by admin

9:20 A – 5:00P, 8:30 P – 12:00A miniprep Taf and Tub samples Register with GeneWiz and send samples for sequencing STORM imaging of en-Epc-H3 continues, focus lock lost early this morning when moving to top of new strip.  Pause … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Friday 04/13/12

Wednesday 04/11/12

Posted on April 11, 2012 by admin

10:00A – 11:30P coating 5o mm coverslips put coverslips in metal, vertical mount holder.  Don’t stay balanced in ceramic holder.  Need to alternate positions or the coverslips stick. Do coating in 400 mL in 1000 mL beaker (not 300 mL … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Wednesday 04/11/12

Tuesday 04/10/12

Posted on April 10, 2012 by admin

10:00A -5:00P, 10:00P – 11:00P Take PCRs out of thermocycler Take bead images from STORM run Check plates from transformation.  Pick colonies tonight if good. All plates have lots of colonies, including negative control :(.  Test gel of PCR cloning

Posted in probe and plasmid building, Summaries | Tagged | Comments Off on Tuesday 04/10/12

Monday 04/09/12

Posted on April 9, 2012 by admin

10:00 A – 10:30 P Lab meeting (Doory) Fix alignment of 750 laser with Colenso’s help Freeze Bcd-GFP and eve-GFP bottles. NuGru presentation (Andi) Working on snail manuscript supplemental figure captions Download ModEncode data for H3K4me3 and H3K27ac Etch and … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Monday 04/09/12

Friday 03/23/12

Posted on March 23, 2012 by admin

10:45 A – 12:30 A O/N confocal 10 um is a little too much buffer between first and last, several embryos didn’t make it all the way through.  Try again tonight. Set up new O/N confocal run 6 um difference … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Friday 03/23/12