Tag Archives: cloning

Friday 05/04/12

12:00 P – 9:30 P 8:30-12P volleyball practice update probe & plasmid library update antibody labels collect virgins set up shRNA crosses x Pc shRNA2 and Psc shRNA 1 in collection cages PCR for in vitro transcription rxn to make … Continue reading

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Tuesday 05/01/12

11:00 A – 6:45P, 8:00P -12:00A flip fly collection cages, 11A Miniprep cultured colonies: 8E, 7E, 2E, AbdB2 and Fab8 (2 colonies each).  Send for sequencing. Rearrange ultratome room, suspend on air-table, clean room. Flip collection cages, 3:15 A Analyzing … Continue reading

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Monday 04/30/12

9:45 A – 8:15P, Steven group meeting presentation sequencing results: AbdB-1A: sense strand.  Use SP6 for antisense probe AbdB-1B: sense strand.  Use SP6 AbdB-3A: sense strand. poor quality read AbdB-3B: antisense strand. Use T7 Dfd-2A: antisense strand: T7 Dfd-2B: antisense … Continue reading

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Friday 04/27/12

10:20A – 5:10P, 9:00P – 11:00P Etch hb-y(i), Bcd sample STORM hb-y(i), Bcd sample remember to turn 642 laser power back up! miniprep colonies: AbdB-1A,B AbdB-3A,B, Dfd-2A,B, 5E-A,B.  all over 100 ng/uL. send 500 ng for sequencing to GeneWiz. move ligation … Continue reading

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Thursday 05/26/12

10:00 A – 11:45 P Check colonies from Bx-C round 1 cloning: all plates look good, no lawns. Gel-extract linearized Taf, Tub Gel-extract newly cloned Bx-C probes Antibody synthesis: antibodies 61-69 Flip collection cages: 10:15 A Washing out primary antibody … Continue reading

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Friday 04/13/12

9:20 A – 5:00P, 8:30 P – 12:00A miniprep Taf and Tub samples Register with GeneWiz and send samples for sequencing STORM imaging of en-Epc-H3 continues, focus lock lost early this morning when moving to top of new strip.  Pause … Continue reading

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Wednesday 04/11/12

10:00A – 11:30P coating 5o mm coverslips put coverslips in metal, vertical mount holder.  Don’t stay balanced in ceramic holder.  Need to alternate positions or the coverslips stick. Do coating in 400 mL in 1000 mL beaker (not 300 mL … Continue reading

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Tuesday 04/10/12

10:00A -5:00P, 10:00P – 11:00P Take PCRs out of thermocycler Take bead images from STORM run Check plates from transformation.  Pick colonies tonight if good. All plates have lots of colonies, including negative control :(.  Test gel of PCR cloning

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Monday 04/09/12

10:00 A – 10:30 P Lab meeting (Doory) Fix alignment of 750 laser with Colenso’s help Freeze Bcd-GFP and eve-GFP bottles. NuGru presentation (Andi) Working on snail manuscript supplemental figure captions Download ModEncode data for H3K4me3 and H3K27ac Etch and … Continue reading

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Friday 03/23/12

10:45 A – 12:30 A O/N confocal 10 um is a little too much buffer between first and last, several embryos didn’t make it all the way through.  Try again tonight. Set up new O/N confocal run 6 um difference … Continue reading

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