9:20 A – 5:00P, 8:30 P – 12:00A
- miniprep Taf and Tub samples
- Register with GeneWiz and send samples for sequencing
- STORM imaging of en-Epc-H3 continues, focus lock lost early this morning when moving to top of new strip. Pause feature of dave fails during reset.
- Started new run in new sub-folder to avoid over-writing. 750 has strong background large features in image. I suspect this is resin because 750 never gives background in my tissue normally.
- washing out primary antibodies in oligo-STORM sample.
- Tiling large regions with Steve on STORM2: select x and y at bottom and press ‘g’ to start tiling. Note x is veritcal span and y is horizontal. System takes 1 image in the middle and then scans from top to bottom building columns left to right.
- Formatting 4TB WD external for RAID (and NTFS): WD icon on windows popup (same as for ejecting USB). Select RAID 1. Check serial number to be sure you are formatting the correct drive if formatting through Tuck / Cajal.
- New sample of en-EPc-H3 etched for 1.5 hrs in new (cold stored, anhydrous) sodium ethoxide solution is switching beautifully and completely etched. No crazy background in 750.
- transformation largely failed, no colonies, including positive control. Repeating ligation with 20 uL volume and 10 uL of gel-extract.
- STORM run 5p – 8P
- O/N STORM run, also en-EPc-H3