Tag Archives: hb

Protected: Drosophila Transcription Projects

Posted on October 30, 2013 by admin

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hunchback and bcd antibody testing

Posted on January 12, 2012 by admin

some quick checks looking at dynamically refinement of hb gradient. Hb (green) and Bcd (red) double stained (early cc13?)         zoom , showing subnuclear localization patterns.           showing very gradual hb transition   … Continue reading →

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Protected: Wednesday 01/11/12

Posted on January 12, 2012 by admin

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Wednesday 07/13/11

Posted on July 13, 2011 by admin

8:35 A — 3:50P, 8:00P – 11:00P Running E(spl)_m8 data and repeat YW Figures for E(spl) and vnd data Printing probe labels (for Levine Lab database) Printing probe template labels (for Levine Lab database) Aliquoting probes. Figures for APtoll APtwi-rm910 … Continue reading →

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Protected: hb cc13 mRNA count summaries

Posted on July 13, 2011 by admin

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Tuesday 07/12/11

Posted on July 12, 2011 by admin

8:15 A — tried high threshold on high consecutive layers for MP06 counts, need to repeat with standard trial set of inputs. Rewriting hb-curve plotting to compute means of multiple gradients (using interp1 so curves can be matched on x-axis). … Continue reading →

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Protected: Lab meeting feedback

Posted on July 11, 2011 by admin

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hb y Hz data summaries

Posted on July 8, 2011 by admin

Set missG to 1 for all data sets. Using raw density instead of size-adjusted counts per cell subtracting maternal contribution post processing as removing lowest cell count. Without maternal correction: 

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Friday 07/08/2011

Posted on July 8, 2011 by admin

8:30 A – 6:00 P ON confocal failed, too much drift. Organizing hb data into lab meeting part 2. writing embryo matching script using wildtype profiles for hb Rewrote anlz_hb_gradient_data.m and Plot_hb_data.m to rely just on raw density rather counts … Continue reading →

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Thursday 07/07/2011

Posted on July 7, 2011 by admin

8:30 A — 4:50 P working on Plot_hb_data: match embryos based on endogenous gradients Reprocessing MP09 data, the .05 for speed on wt is a little too high, causes some dot loss, drop to a more conservative .03 or .01. … Continue reading →

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