hunchback and bcd antibody testing

some quick checks looking at dynamically refinement of hb gradient.

Hb (green) and Bcd (red) double stained (early cc13?)

 

 

 

 

zoom , showing subnuclear localization patterns.

 

 

 

 

 

showing very gradual hb transition

 

 

 

 

hb (after applying mask), used in calc above. mask is much easier to make using Cm-diI inverse than using a nuclear dye.

 

Bcd (after applying mask)

 

nuclear dye labeled cycle 14 embryo (note nuclear density).

bcd red (blue trace below), hb green.

note much sharper transition in cycle 14 embryos

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