hunchback and bcd antibody testing

some quick checks looking at dynamically refinement of hb gradient.

Hb (green) and Bcd (red) double stained (early cc13?)





zoom , showing subnuclear localization patterns.






showing very gradual hb transition





hb (after applying mask), used in calc above. mask is much easier to make using Cm-diI inverse than using a nuclear dye.


Bcd (after applying mask)


nuclear dye labeled cycle 14 embryo (note nuclear density).

bcd red (blue trace below), hb green.

note much sharper transition in cycle 14 embryos

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