Tag Archives: embryo labeling

Thursday 07/25/13

STORM break down O/N run of R2bxcD STORM analysis run all bxc data dot-fitting (que up on Tuck in small batches, Cajal not talking) run all bxc mosaic file conversions (que up on Cajal in linear batches) Embryo staining incubating … Continue reading

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Tuesday 06/04/13

10:00 A – 12:30 A cell staining heating wash buffers to 47C imaged 20 bp single probe cells (5 cells). imaged 40 bp array (with 0 gain, oops). (6 cells) imaged 40 bp array for restain (7 cells) Doory imaging … Continue reading

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Monday 05/20/13

9:20 A – 7:00P, 9:30P — 12:20 A Lab meeting visiting post-doc candidate: notes Probe making figure out increased library concentrations for large scale PCR for B/Y/K/G libraries set up 5 mL scale PCR for B/Y/K/G using nick-containing primers Setup … Continue reading

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Sunday 03/03/13

10:00A – 1:00P, 6:00P – 7:45P, 9:00P – 11:00P Embryo Section staining Control test with AATAT worked. basic protocol No 92C pre-denature 60C 25 min minimal cooling gap. Add probe to slide, drop coverglass on, seal 94C heat-block 2.5 min … Continue reading

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Friday 01/02/2013

9:45 A – 7:15P, 8:30P – 10:40 P STORM Record z-calibration curve for STORM3 Finish O/N run of Fab7 on STORM3 Finish O/N run of BX-C embryos on STORM2. Ideal focus plane not matched between regions (this can be custom … Continue reading

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Thursday 01/31/13

10:45 A – 12:30 A (8:45-10:45 workout) embryo section labeling quick check after 10 min hot wash in incubator at 70C in SSCT, found no sections. DAPI staining sections. Try again. weak BXC detectable: Prepped for STORM TRY 2: prepping … Continue reading

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Tuesday 12/18/12

11:00 A -7:30P, 9:15P-9:55P Cell culture S2 cells in chamber wells too overgrown S2 cells in 75 cm^2 plates, in need of passage s2R+ in chamber wells look good for fixation.  Not too spread out despite polyL though. S2R+ in … Continue reading

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Monday 12/03/12

8:45 A — 7:30 P, 8:45P – 12:40 A Confocal data Finish O/N confocal run transfer files modify imviewer LSM to automatically detect all .lsm files in a folder and convert them all to TIF / labeled stacks of TIFs … Continue reading

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Sunday 12/02/12

10:30 A – 7:30P, 8:30P – 11:45P Lab meeting prep collecting dual labeled images to show attempts with flanking probes. PCR Test run gels from Espl screening.  Gel 1 from BioXAct (3A and 4A, see legend in yesterday’s post), numerous … Continue reading

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Saturday 12/01/12

cell staining control worked, 50kbp probe did not. However, 50kbp probe does show weak global nuclear staining immediately after removing probe, which is not visible in control channels. hot washes started 60C SSCT hot washes dramatically reduce signal from control … Continue reading

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