Tag Archives: PCR

oligopaint-PCR

Posted on April 4, 2013 by admin

Calculating concentrations (Qubit) and molecule numbers: starting primer = 30 ng in 300 uL in 1 ng/uL, added 100 uL = 100 ng 100 uM = 100E-12 mols/uL add 100 uL, = 10 nmols starting primer. Y PCR: 73.7 ng/mL … Continue reading →

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Sunday 12/02/12

Posted on December 2, 2012 by admin

10:30 A – 7:30P, 8:30P – 11:45P Lab meeting prep collecting dual labeled images to show attempts with flanking probes. PCR Test run gels from Espl screening.  Gel 1 from BioXAct (3A and 4A, see legend in yesterday’s post), numerous … Continue reading →

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Friday 11/30/12

Posted on November 30, 2012 by admin

9:30A – 10:15 P Fly Work Collect sna[18] x Double balancer virgin and male F1s PM virgin collection STORM2 time. Colenso removing gas laser from STORM2 Mount ultra cryo section embryo for imaging sections not flat or hard to find. … Continue reading →

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Thursday 11/29/12

Posted on November 29, 2012 by admin

9:20 A — 7:15P, 8:15P – 10:00P Polytene cell Check polytene cell staining.  Faint glow of nuclear laminin visible.  pretty dim, does not have ring shape, see top and bottom (would be easier to distinguish as diffinitive stain by confocal). … Continue reading →

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