Tag Archives: probe making

Tuesday 07/22/14

11:25 am — 11:45 pm (biking Minuteman path, 8:15a-11:25a) Goals Ajaz’s sample project 2 data analysis chromatin data analysis Project 2 analysis see protected post Chromatin Project Data analysis fitting E06 and G2toG4 data trying to analyze BX-C data from … Continue reading

Posted in Summaries | Tagged , | Comments Off on Tuesday 07/22/14

Monday 06/02/14

9:45am – 4:45pm, Goals Set up RT reactions Lib3 samples B10-B12, C09-C12, D01-D08 Wash newly stained samples (B08, B09, E11-F01, + PH immuno-FISH) set up STORM imaging of PH immuno-FISH Lab Meeting see notes General letterhead and signature for letter … Continue reading

Posted in Summaries | Tagged , | Comments Off on Monday 06/02/14

Wednesday 04/09/14

9:30a – 8:30p Chromatin labeling stains failed again. No stains yet detected with this batch of cells. maybe it’s a cell prep issue? This F07 probe worked fine before. maybe some reagents went bad in the cell prep? Make new … Continue reading

Posted in Summaries | Tagged , , | Comments Off on Wednesday 04/09/14

Saturday 03/29/14

10:15a – 12:15a Multi-color data analysis Analyzing chromatic bead data from 2014-02-27 and 02-28 Analyzing drift bead data from 2014-02-27 / 28 should write a little script to auto launch this stuff? 2014-02-28 F06F05 dataset, Image 25 and on, Conventional … Continue reading

Posted in Summaries | Tagged , | Comments Off on Saturday 03/29/14

Friday 03/28/14

9:45a – 11:50p Goals Record bead fields. check isoforms differences and consequences for probes selected (do our probes still hit the most popular isoform of imr90s?) Cell Staining washout stains for new multi-color samples. Need to wait until oven is … Continue reading

Posted in Summaries | Tagged , , | Comments Off on Friday 03/28/14

Thursday 04/27/14

10:15a – 12:30a Goals Data processing copy C05 and C06 data to hard-drives Launch analysis of C03-C06 data plate, fix, prep and hybe new cells Probe making RT reactions test gel probe purfication cell staining wash out probes image multi-color … Continue reading

Posted in Chromatin | Tagged , , , , | Comments Off on Thursday 04/27/14

Wednesday 03/26/14

9:20a – 10:10p, remotely, 10:50p – 11:30p Goals passage cells washout probe from new multicolor stain finish updating notes from yesterday STORM run configurations data analysis launched break down PRISM2 run of L3C04. (complete) break down STORM2 run of L3C03 … Continue reading

Posted in Summaries | Tagged , , , , | Comments Off on Wednesday 03/26/14

Sunday 12/29/13

9:00a – 12:15a Goals Write to Ajaz about new plans Read in _oligo.txt files and analyze probe distributions STORM G1-G2 Start analysis of F12-G01 and F12-G11 data continue work on STORMrender Manual Literature Reading Kieffer-Kwon et al Cell 2013. link … Continue reading

Posted in Summaries | Tagged , , , , | Comments Off on Sunday 12/29/13

Sunday 12/22/13

9:45a – 11:50p Goals align 750-647 G1 double stains matlab-storm STORMrender update menu options for feducial drift correction to allow finding the bin file of feducial data using the browser (oops, this was already the default for an empty input, … Continue reading

Posted in Summaries | Tagged , | Comments Off on Sunday 12/22/13

Wednesday 12/18/13

10:30a – 8:15p, 12:00p- 1:00a Meeting with XZ prepping slides (see notes) Test probes synthesis analyze tomorrow Coding matlab-storm Work with Jeff to restructure startup / installation need to update readme need to update all global variables to new conventions … Continue reading

Posted in Summaries | Tagged , , | Comments Off on Wednesday 12/18/13