10:15a – 12:15a
Multi-color data analysis
- Analyzing chromatic bead data from 2014-02-27 and 02-28
- Analyzing drift bead data from 2014-02-27 / 28
- should write a little script to auto launch this stuff?
- 2014-02-28 F06F05 dataset, Image 25 and on, Conventional no longer aligns with STORM image :(. Still have 50+ excellent images.
- 2014-02-27 F04F05 dataset — 647 F5 very poor switching, few localizations. Overlap still looks reasonable.
- worth analyzing but also worth repeating.
- Set up new RNase free area in upstairs lab with new dedicated pipettes.
- E01 – E05 with P1 (tubes 1-5)
- E03, E04, E05 also with P3 (tubes 6-8)
- E06 – E08 failed PCR, try again in a later batch.
- Out of P3-405!
- loading order E01-E05 P1, then E03-E05 P3.
Final probe concentrations
Ph Project, to do:
- Make supplement image of antibody controls
- write caption for supplement image of antibody controls
- write caption for supplement image of molecule counts (instead of diameter)