Tag Archives: snail

Protected: Drosophila Transcription Projects

Posted on October 30, 2013 by admin

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Monday 11/19/12

Posted on November 19, 2012 by admin

9:15 A — 7:15 P, 8:15P – 1:15 A Cell labeling hot washes for probe first slide (2x at 60C) 1x at RT remove and save primary antibodies at RT for primary first slide. PBT rinses (3x soln change, 3rd … Continue reading →

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Protected: analyzing promoter swap data

Posted on August 6, 2012 by admin

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Wednesday 07/04/12

Posted on July 4, 2012 by admin

12:30 P – 6:45P, 7:45P – 10:10P wash out primary antibodies. dispatched old reserves of dilute, reused primary antibodies.  Moved new batch to freezer.  Will test stain later — should preserve better for one extra round. Test DNA-FISH probes.  centromeric … Continue reading →

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Tuesday 05/29/12

Posted on May 29, 2012 by admin

1:00 P – 4:00P, 9:00P-12:00A running insight analysis on K4 K27 data  (11:00A) using only single iteration for computation of z-calibration curve. compare structure of K4, K27me3 and K27ac chromatin (?) troubleshooting issue with non-convergence of z-calibration bead data by … Continue reading →

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Protected: snail papers

Posted on May 29, 2012 by admin

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Protected: snail dose effects

Posted on May 28, 2012 by admin

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Thursday 05/10/12

Posted on May 10, 2012 by admin

10:00A -8:00P working on chromatic correction for beads to apply to molecule lists Finished script to merge multiple bead fields from confocal and compute transforms. working on intro sections of ESF talk, formatted to work on laptop. comments back from … Continue reading →

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