Tag Archives: communication

Protected: Ph Polymerization: on modeling

Posted on April 1, 2014 by admin

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Thursday 03/13/14

Posted on March 13, 2014 by admin

9:00a — 11:30p communication Help Jaeger student with Unsupervised_Dotfinding.m (see email) meetings planning meeting for G0 presentation jeff will embed movies got slide from Bryan got movies from Colenso put in example images of chromatin need to rehearse talk! meeting … Continue reading →

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Wednesday 12/11/13

Posted on December 11, 2013 by admin

11:15a – 11:45p Ph Project Highly variable radius of gyration measurements for case with binding — suggests not equilbirated. Binding strength probably much to high. Up to .90, run again. Also increased density of K27me3 sites from 50% to 80%, … Continue reading →

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Thursday 10/03/13

Posted on October 3, 2013 by admin

10:00a – 7:00p, 10:00p – 11:50p Goals today prep slides for meeting with XZ 10 cycle PCR with 2 uL of product to add on NEB adapters STORM of more black regions reply to Nicole and Ajaz about Ph Literature … Continue reading →

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Friday, 12/07/12

Posted on December 7, 2012 by admin

9:45 A – 8:20 P Stop O/N confocal Prep for ultra sectioning Discussion with W. Bender Graded AbdB expression probably produced by combinatoral enhancer action (multiple enhancers leading to the stronger pattern) early enhancers are somewhat segment like.   Not … Continue reading →

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Wednesday 11/14/12

Posted on November 14, 2012 by admin

9:00A – 7:30P, 8:15P – 11:15P Prepping gel (low on Na Borax, and TAE making up new TAE) Test gel: YW postive control AbdB looks great.  YW negative control sim[D] is clean.  Improvised gel extractions not looking good. Try sim[D] … Continue reading →

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Wednesday 11/06/12

Posted on November 7, 2012 by admin

9:45 A – 8:30 P write to ML about bistability write to Bender about Hox model.  — apparently email contact is not recommended.  Try phone tomorrow. write summary of Francis suggestions remove medium from S2 cells, add fresh medium.  Reduce … Continue reading →

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Monday 09/17/12

Posted on September 17, 2012 by admin

9:30 AM – 7:30PM, 9:00P – 11:00P Start Unsupervised_Dotfinding for mRNA counting of sog data. Troubleshooting error with list2img for movie2vectorSTORM: not outputting Gaussian image Getting GPU gaussian blobs script to work — test script works great Lab meeting (Shu: … Continue reading →

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Thursday 09/13/12

Posted on September 13, 2012 by admin

9:00 A – 4:00P, + remotely 5:00P-7:20P, 8:20P-11:20PM O/N STORM run still going smooth and in focus at 8:00 AM. O/N STORM run completes on time, 9:30AM.  Break down run turn off scope. Coding Coding matlab wrapper for python / … Continue reading →

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Friday 09/07/12

Posted on September 7, 2012 by admin

9:05 A – 8:00 P O/N STORM lost focus attempt to get bead images aborted.  Issues with focus lock delayed start and Colenso needs to get on the machine. O/N confocal still had lots of issues with z-focus (apparently not … Continue reading →

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