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Tag Archives: sectioning
Tuesday 01/22/12
Fly Work collect virgins (df/CyO-Esc[2]) AM # New crosses with remaining MTDs! # collect virgins PM Ultra-cryo sectioning yw embryos Cutting sample at -51C, knife at -54C, TG -50C at .65 – .75 um cuts pretty well, still better … Continue reading
Friday, 12/07/12
9:45 A – 8:20 P Stop O/N confocal Prep for ultra sectioning Discussion with W. Bender Graded AbdB expression probably produced by combinatoral enhancer action (multiple enhancers leading to the stronger pattern) early enhancers are somewhat segment like. Not … Continue reading
Friday 11/02/12
9:30 A – 7:48 PM, 10:50 – 11:45PM Fly work Flip collection plates on MTD x SCM, MTD, and sim[D], 9:30A Flip collection plates, sim[D] 1:15 P Collect virgin Act5-Gal4, MTDs, and sim/Espl. Sort sim/Espl against humoral bristles. New crosses … Continue reading
Posted in Fly Work, probe and plasmid building, Summaries
Tagged fly work, probe making, sectioning
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Tuesday 09/11/12
9:10 AM – 7:15 P, 9:00P – 11:55 P Log off Tuck (Reserved for Yari) – 7 Processes all doing final fit on ~500,000 molecule list (about 1 molecule/per sec! These are gonna have to get killed…) Finish O/N STORM … Continue reading
Wednesday 09/05/12
9:30 A – 9:00 P sectioning 6/25 sample 10: E(Pc)-dig, en-bio, H3-750, K27-647. writing figure text for snail promoter/enhancer manipulations First coverslip of S10 most sections adhered to coverslip before chloroform treatment. may not be fully flattened. 4 coverslips cut, … Continue reading
Tuesday 09/04/12
9:30A -4:00P Take bead images for color calibration. (looks like system may have relaxed and is now clipping on the right instead of on the left? Check when next I have scope time… Temporal analysis of snail dynamics with different … Continue reading
Wednesday 08/28/12
9:50 A-7:00P, 8:30P – 11:50P pick up drive from HCBI STORM4 lost focus O/N. Reset focus, continue imaging. Trying to get good matches for pnrPr dynamics in simulation, not just end states. Dyes not switching well in 15 min etched … Continue reading
Thursday 07/26/12
10:20 A New fly cages of Pc-GFP and yw. Pc-GFP not growing well, should have ~10 bottles/cage. 4 would be sufficient for yw cage. Crossed virgin 3x mat Gal4 x Pc-shRNA2 in vial test viability. new bottle of 3x mat … Continue reading
Posted in Summaries
Tagged DNA-FISH, genomics, in situs, sectioning
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Wednesday 02/25/12
10:00 A – 7:30P, 8:50P – 12:15 A checking sna-y counts. Rerunning analysis scripts on MP10 and MP05 data seems to me MP05 have more counts than MP10 in both 2012-02-17 data and 2012-06 and 2012-05 datasets. Not consistent … Continue reading
07/08/12
3:30 P – 6:10 P Trimming H3K4me3 4-color, H3, and Dm01 4-color labeled samples.