Tuesday 01/22/12

 

Fly Work

  • collect virgins (df/CyO-Esc[2]) ¬†AM
  • # New crosses with remaining MTDs!
  • # collect virgins PM

Ultra-cryo sectioning yw embryos

  • Cutting sample at -51C, knife at -54C, TG -50C
  • at .65 – .75 um cuts pretty well, still better at .65.
  • at 1 – 2 um sample is very crumbly and likes to curl quite a lot.
  • cutting 1 um sections on glass knife at -50C for all components.

Probe Making

  • Precipitate and resuspend En probe.

Round 1 in situs with BX-C and en in S2 Cells (Brian and Chamith)

BXC_3Cy5sec_confocal_field

BXC_3Cy5sec_confocal_field

BXC_3Cy5sec_confocal_zoom

BXC_3Cy5sec_confocal_zoom

BXC_no_sec_zoom_confocal

BXC_no_sec_zoom_confocal

Engrailed_3Cy5_secondary

Engrailed_3Cy5_secondary

Engrailed_no_secondary

Engrailed_no_secondary

BXC_3Cy5_secondary

BXC_3Cy5_secondary

BXC_no_sec_field

BXC_no_sec_field

BXC_no_secondary

BXC_no_secondary

 

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