- collect virgins (df/CyO-Esc) AM
- # New crosses with remaining MTDs!
- # collect virgins PM
Ultra-cryo sectioning yw embryos
- Cutting sample at -51C, knife at -54C, TG -50C
- at .65 – .75 um cuts pretty well, still better at .65.
- at 1 – 2 um sample is very crumbly and likes to curl quite a lot.
- cutting 1 um sections on glass knife at -50C for all components.
- Precipitate and resuspend En probe.
Round 1 in situs with BX-C and en in S2 Cells (Brian and Chamith)