10:00 – 8:30P, 9:30P-11:00P
(9A-10A workout session)
10A-1:30P lab meeting George. Notes
Fly work
- MTD crosses
- Psc-1 parental cross started at 29C gives viable F1s.
- Psc-2 no F1 progeny — (need to redo with fresh food + more flies)
- Rad21 parental cross start at 29C gives only 5 males. female lethal/skewed/random?
- Su(Z)12 started at 29C, gives only 2 females (no good for F1xF1)
- SCM started at 29C has no progeny. SCM at 22C now crossing F1 at 29C
- Pc-2: P started at 22C gives F1. now crossing at 29C
- ph-D: cleared 2 vials ready to emerge F1s.
- screening backcross Espl sim
- [spreadsheet 0AjSqkxgziU1YdENuMzFjejM0TnNXUEQ1OWdHRW1OY1E 602 302 sheet = 1]
- Selected Hu males for 10 more vials (all of row C).
- cross to Espl[D] and sim[D] virgins.
- Clear Esc[6]/CyO x Sp/CyO vials. Collect Esc[6]/Sp males
- Cleared df/CyO-Esc[2] bottles. Moved to 18C for virgin collection. Collect tomorrow
- 2x Mat alpha-Tub almost ready to move to 18C to start virgin collection.
- Finish flipping fly stocks (orange rack).
- successfully made sna[18]/CyO-LacZ; Tm2/Tm6 (ebony). should confirm with Valerie that these are CyO-LacZ.
Probe making
- 1-butanol extract redisolved gel extracted ssDNA-cy5 probe
- ~200 uL concentrated probe each.
- let excess T-butanol evaporate
- add 5 uL glycogen 22 uL 4M NH[4]OAc, 1 mL cold 100% EtOH.
- precipitate at -20C 2 hrs
Coding
- Test and troubleshoot few bugs in RunDotFinder.m (as called from STORMfinder) on InsightM files.