Monday 01/21/13

10:00 – 8:30P, 9:30P-11:00P

(9A-10A workout session)

10A-1:30P lab meeting George. Notes

Fly work

  • MTD crosses
    • Psc-1 parental cross started at 29C gives viable F1s.
    • Psc-2 no F1 progeny — (need to redo with fresh food + more flies)
    • Rad21 parental cross start at 29C gives only 5 males.  female lethal/skewed/random?
    • Su(Z)12 started at 29C, gives only 2 females (no good for F1xF1)
    • SCM started at 29C has no progeny.  SCM at 22C now crossing F1 at 29C
    • Pc-2: P started at 22C gives F1.  now crossing at 29C
    • ph-D: cleared 2 vials ready to emerge F1s.
  • screening backcross Espl sim
    • [spreadsheet 0AjSqkxgziU1YdENuMzFjejM0TnNXUEQ1OWdHRW1OY1E 602 302 sheet = 1]
  • Selected Hu males for 10 more vials (all of row C).
  • cross to Espl[D] and sim[D] virgins.
  • Clear Esc[6]/CyO x Sp/CyO vials.  Collect Esc[6]/Sp males
  • Cleared df/CyO-Esc[2] bottles.  Moved to 18C for virgin collection.  Collect tomorrow
  • 2x Mat alpha-Tub almost ready to move to 18C to start virgin collection.
  • Finish flipping fly stocks (orange rack).
  • successfully made sna[18]/CyO-LacZ; Tm2/Tm6 (ebony).  should confirm with Valerie that these are CyO-LacZ.

Probe making

  • 1-butanol extract redisolved gel extracted ssDNA-cy5 probe
  • ~200 uL concentrated probe each.
  • let excess T-butanol evaporate
  • add 5 uL glycogen 22 uL 4M NH[4]OAc, 1 mL cold 100% EtOH.
  • precipitate at -20C 2 hrs

Coding

  • Test and troubleshoot few bugs in RunDotFinder.m (as called from STORMfinder) on InsightM files.
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