Sunday 01/20/13

11:00 A – 4:00P, 10:00P-12:00A

Coding

  • Frame-by-frame analysis now runs in a platform independent way form the new shared project matlab-storm folder for both DaoSTORM and InsightM
  • Writing new Analysis script (RunDotFinder) — modified, more flexible version of Run_Insight that takes inputs to do either InsightM or DaoSTORM
  • still needs testing

Probe Making

  • Cast new  12% polyacrylamide gel
  • Gel extract single stranded cy-5 labeled En probe.  Dissolving crushed pellet in ddH2O at 37C O/N following BH’s denaturing PAGE protocol (see Protocols page).
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