Tag Archives: in situs

Saturday 12/01/12

Posted on December 1, 2012 by admin

cell staining control worked, 50kbp probe did not. However, 50kbp probe does show weak global nuclear staining immediately after removing probe, which is not visible in control channels. hot washes started 60C SSCT hot washes dramatically reduce signal from control … Continue reading →

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Monday 11/12/12

Posted on November 12, 2012 by admin

9:45 A – 7:30 P, 8:45P – 10:00P Troubleshooting issues with bead warping wrote short translation correction to (1D polywarp) to correct the gross aberrations introduced by the STORM4 quadview. Still seems to be a shift.  Might be due to … Continue reading →

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Saturday 08/04/12

Posted on August 4, 2012 by admin

10:00A-2:45P, 5:20P-7:00P, 8:30P-10:30P in situs day 3 (triple stain try 2). STORM imaging slide 2 from 6/24 stains (Antp) mount samples 1-5 + centromere/K27/Dm01 v2, post-fix rest. Hal running smoothly. Imaging conventional images of each section just prior to STORM … Continue reading →

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Friday 07/27/12

Posted on July 27, 2012 by admin

9:40 A – 7:40, 9:00P – 12:30A Finished confocal run, 8.5 embryos finished at speed 5 by 10A (run started at 10P). Previous user canceled 710 time (again).  Need to check before starting or stopping a run. Over-etched first K27 … Continue reading →

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Thursday 07/26/12

Posted on July 26, 2012 by admin

10:20 A New fly cages of Pc-GFP and yw.  Pc-GFP not growing well, should have ~10 bottles/cage.  4 would be sufficient for yw cage. Crossed virgin 3x mat Gal4 x Pc-shRNA2 in vial test viability. new bottle of 3x mat … Continue reading →

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Tuesday 07/10/12

Posted on July 10, 2012 by admin

10:10 A – 5:15 P, 10:30P – 12:45A etching H3K4 4-color coverslip 3 in new sodium ethoxide (pale yellow color) for 13 min = mostly etched, resin definetely in the doubled over sections, some patches on single embryos.  clear bur … Continue reading →

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Monday 07/09/12

Posted on July 9, 2012 by admin

9:50 A – 6:50 P remove slide 3 of H3K4-section from 60C heat block, add to slides. Hazen Lab meeting 3D viewing in Insight:  ctrl+shift+x, select ‘no’, ctrl+x gives 3D viewer.  Need to have color shading as percentage toggled on … Continue reading →

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Wednesday 06/27/12

Posted on June 27, 2012 by admin

10:10 A – 11:00P in situs day 3 washing out and post-fixing Pc labeled primaries write to Nicole Follmer about antibodies lit review: piRNA in C elegans multigenerational epigenetic memory.  LacR facilitated diffusion.  DNA sequence dependent compartmentalization and silencing an … Continue reading →

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Tuesday 06/26/12

Posted on June 26, 2012 by admin

10:30 A -5:00P, 9:00P – 11:30P in situs day 2 short fix 2-14 hour yw and Pc embryos. rehydrate and stain 2-14 hour short fix embryos with Su(Z)12.  Pc-GFP embryos also stained with ch a-GFP. vball lost. Synthesized Bac 1 … Continue reading →

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Monday 06/25/12

Posted on June 25, 2012 by admin

9:45 A – 7:00P, 9:30P – 11:45P Need new collection cages FISH + K27 on O/N embryos day 1 Flipped 3x Mat Gal4 cages. Running Z-calibration analysis on Suz647 data (with Quadview) Collecting new z-cal bead data in 512 and … Continue reading →

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