Thursday 07/26/12

10:20 A

  • New fly cages of Pc-GFP and yw.  Pc-GFP not growing well, should have ~10 bottles/cage.  4 would be sufficient for yw cage.
  • Crossed virgin 3x mat Gal4 x Pc-shRNA2 in vial test viability.
  • new bottle of 3x mat Gal4.
  • 2 bottles of 3x mat Gal4 ready for virgin collection, now at RT in fly room.
  • Need to add water to 3x mat Gal4.
  • Final revisions to kinetics paper / letter for NSMB
  • Submitting kinetics paper to NSMB
  • Etch yw coverslip – staining with Hoechst to test DNA.
  • attempting on-coverslip-staining and hybridization with centromeric probes.
  • Sectioning: K27-cntrls.
  • Sectioning: centromere-FISH controls.  Check by Turnkey — DNA FISH signal survives embedding.  Not especially common among nuclear sections.
  • water not sticking to edge of knife.  Great difficulty sectioning centrome-DNA-FISH samples.
  • Running analysis of MP06 data (with revised version of dot counting code from final revision of snail levels study): for use in bistability paper.
  • In situs day 2:
  • [spreadsheet 0AjSqkxgziU1YdG90a29UaU5ua1FLUXZ5LTFmM1pMSlE 602 302 sheet=8]
  • Attempting to analyze H3K27me3 data in ESC mutants (from Vivek’s paper) with Galaxy.
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