10:00 A – 7:30P, 8:50P – 12:15 A
- checking sna-y counts. Rerunning analysis scripts on MP10 and MP05 data
- seems to me MP05 have more counts than MP10 in both 2012-02-17 data and 2012-06 and 2012-05 datasets. Not consistent with rescue observations or previous characterization, labels maybe switched? should repeat?
- KE rehydrated embryos for new in situs in 70% EtOH and used 70% EtOH for the move to xylenes. Embryos clump awkwardly. Attempting to save in situ by transfering to 100% EtOH and repeating xylene treatment.
- request from Bio Phys J for review.
- Trim for sectioning, embedded embryos:
- yw (unstained, no GA fix)
- K27-controls
- Pc-647 Pc-GFP cntrl
- Pc-750 Pc-GFP cntrl
- centromere-647 STORM
- Sectioned embryos:
- Pc-647 Pc-GFP cntrl
- yw (unstained) — etch and use for FISH on coverslips.
- Observations: thin short sections make much better ribbons. some issues still with water clinging to knife. left half has two very small burs that cut ribbon into pieces. Center left has a larger bur which creates abberations in cut section.
- write letter to editor for NSMB, send to Stas
- # Set up new Pc and yw cages!