Protocols

Version Managed Protocols:

• These links direct to the most recent updated version of the indicated protocol (hosted on Github)
• The protocol update history can be viewed on Github. Links in individual posts in notebook direct to the version of the protocol as it stood on the day it was performed.

OligoPaints Related

• emulsion PCR
• Oligo Probes Synthesis Protocol
• STORM FISH hybe Protocol
• OligoPaint hybe on tissue cryo-sections
• Dye Labeling of Nucleic Acids
• HPLC purification of labeled DNA

STORM Related

• STORM buffers
• Subbing coverglass

Drosophila embryo labeling

• Alistair’s Embryo in situ’s
• single molecule RNA FISH

General Molecular Biology

• BioRad mini-denaturing gels
• S2 cell culture

 

Old Versions

• These protocols are hosted on wordpress which does not allow permalinks to previous versions of since updated posts.

RNA probe making

• synthesis of RNA in situ probes

Drosophlia Protocols

• RNA in situ hybridization on embryos
• RNA in situ hybe buffers