Category Archives: AP patterning

hunchback and bcd antibody testing

some quick checks looking at dynamically refinement of hb gradient. Hb (green) and Bcd (red) double stained (early cc13?)         zoom , showing subnuclear localization patterns.           showing very gradual hb transition   … Continue reading

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Protected: MP09 intensity sensitivity

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Protected: bcd 1x v 6x cont

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Protected: Analysis of 5-22 data b

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Protected: new hb analysis

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hb-gradient analysis

Analyzing hb-gradient: anlz_hb_gradient_data.m   (committed to Git). Circular field lines not appropriate (obviously).  Need to compute planes of separation instead. May be better to ensure original data has appropriate left-right planar orientation.  This will not only make segmentation easier, but … Continue reading

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Protected: hb enhancer modeling

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Comparison to Gregor data

To count all 770 molecules in the nucleus according to Thomas data (D =1, c=4.8, a=.003) would take 890 minutes (and give 3.6% diffusion error). naturally 100 molecules gives a std/mean 0f 10%, and is thus the natural threshold for … Continue reading

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Protected: Comparison to hb data

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Protected: hunchback modeling

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