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Category Archives: AP patterning
hunchback and bcd antibody testing
some quick checks looking at dynamically refinement of hb gradient. Hb (green) and Bcd (red) double stained (early cc13?) zoom , showing subnuclear localization patterns. showing very gradual hb transition … Continue reading
Posted in AP patterning, Microscopy
Tagged bcd, confocal, hb
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Protected: MP09 intensity sensitivity
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Posted in AP patterning, Microscopy, Shadow Enhancers
Tagged bcd, controls, hb, mRNA counting, shadow enhancers
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Protected: bcd 1x v 6x cont
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Posted in AP patterning
Tagged analysis, bcd, hb, mRNA counting
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Protected: Analysis of 5-22 data b
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Posted in AP patterning, Shadow Enhancers, Transcription Modeling
Tagged bcd, hb, mRNA counting, noise, shadow enhancers
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Protected: new hb analysis
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Posted in AP patterning, Microscopy, Shadow Enhancers
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hb-gradient analysis
Analyzing hb-gradient: anlz_hb_gradient_data.m (committed to Git). Circular field lines not appropriate (obviously). Need to compute planes of separation instead. May be better to ensure original data has appropriate left-right planar orientation. This will not only make segmentation easier, but … Continue reading
Posted in AP patterning, Microscopy
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Protected: hb enhancer modeling
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Posted in AP patterning, Shadow Enhancers, Transcription Modeling
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Comparison to Gregor data
To count all 770 molecules in the nucleus according to Thomas data (D =1, c=4.8, a=.003) would take 890 minutes (and give 3.6% diffusion error). naturally 100 molecules gives a std/mean 0f 10%, and is thus the natural threshold for … Continue reading
Posted in AP patterning, Transcription Modeling
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Protected: Comparison to hb data
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Posted in AP patterning, Transcription Modeling
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Protected: hunchback modeling
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Posted in AP patterning
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