Tag Archives: DNA-FISH

Thursday 10/18/12

Posted on October 18, 2012 by admin

9:40 A – 7:00P, 8:20P – 10:45P Flip collection cage, 9:45A.  Keep plate to make new bottles Pick up more 10x TAE and 20x SSC from stock room Picked up 6 new pints of 100% EtOH. Found issue with K27:H3 … Continue reading →

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Thursday 07/26/12

Posted on July 26, 2012 by admin

10:20 A New fly cages of Pc-GFP and yw.  Pc-GFP not growing well, should have ~10 bottles/cage.  4 would be sufficient for yw cage. Crossed virgin 3x mat Gal4 x Pc-shRNA2 in vial test viability. new bottle of 3x mat … Continue reading →

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Tuesday 07/03/12

Posted on July 3, 2012 by admin

10:00 A – 7:10P, 8:45P-11:00P Sent version 4 of snail dynamics manuscript to ML Flip collection cages. 12:30 PM fix Pc-GFP and yw embryos.  15 min fix.  Rehydrating embryos to test protein stains. Image sections on Turnkey.  sections substantially non-flat. … Continue reading →

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Saturday 10/22/11

Posted on October 22, 2011 by admin

3:00P – 7:00P, 11:00P – 12:00A test gel for repeat trial of nicked translation — still no DNA detectable on gel, despite DNA detectable on spec. Trying straight gel of original isolated DNA — still no band test PCR of … Continue reading →

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