Saturday 10/22/11

3:00P – 7:00P, 11:00P – 12:00A

  • test gel for repeat trial of nicked translation — still no DNA detectable on gel, despite DNA detectable on spec. Trying straight gel of original isolated DNA — still no band
  • test PCR of nicked translation product and test restriction digest with PacI original DNA.
  • loading chromatin condensation image data from s04 and s06 test images from 6/22/11 slides into matlab.
  • install digiKam as an open source 16-bit image reading program. ┬áNeeds to index image files first. ┬áConfirmed Picasa does not support 16 bit images.
  • request secondary antibodies to make new STORM dyes: (donkey anti- goat, sheep, mouse and rabbit) and phase lock gel columns to try alternate BAC extraction.
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