10:10 A – 11:00P
- in situs day 3
- washing out and post-fixing Pc labeled primaries
- write to Nicole Follmer about antibodies
- lit review: piRNA in C elegans multigenerational epigenetic memory. LacR facilitated diffusion. DNA sequence dependent compartmentalization and silencing an nuclear lamina.
- Getting odd error in loading bin files in fxn_fit3Dbeads when called from parent fxn in movie2vectorSTORM. When run independently from the function after error it runs fine.
- writing snail bistability manuscript — new theme on mechanisms of transcription from single cell studies. Reread literature on smFISH. Provide short review of current models of different transcriptional modes.
- Raj 2010 C elegans CoVs are only for end-1 and only computed between 2 cells. Not very robust. Using Shalev’s mouse intenstine data instead — many more genes, higher CoVs (.6 to 4).
- Modified script to deal with errors in list reading without having to re-run list writing.
- froze and cleaned old yw and Pc-GFP cages.
- antibodies to label: dk a-shp Cy3B. dk a-m Atto488 (with new R&D antibodies: dk a-m 750, dk a-rb 750