9:50 A-7:00P, 8:30P – 11:50P
- pick up drive from HCBI
- STORM4 lost focus O/N. Reset focus, continue imaging.
- Trying to get good matches for pnrPr dynamics in simulation, not just end states.
- Dyes not switching well in 15 min etched K27me3. Especially A647. Also has obvious glowing chunks.
- Etching K27 Pc large slide from ~4/20. 20 min etch, still solidly embedded. Etching another 20 min.
- fixed bug in lsm_tif_2_mytif.
- 6 rounds of 20 min etches, K27 Pc etching complete. Manual check confirms nice A647 switching.
- Tiling K27 Pc large slide for O/N STORM imaging.
- Solved issue with 647 not switching –> 405 was up to 10 mW, completely largely bleached 647 channel prior to imaging during the 750 run. Set down to 1.2 mW. Recalibrating Pc K27. Should re-image the Pc-GFP… 🙁
- interestingly almost same proportional increases in dot intensity with the 10x weaker 405.
- sectioned 3 coverslips of slide 1 (AbdB AbdA Ubx).
- sectioned 4 coverslips of centromere stained and counter-labeled nuclei.