Tag Archives: STORM2

Tuesday 04/29/14

Posted on April 29, 2014 by admin

9:30a – 10:00p Computer Cajal troubleshooting computer behaving very sluggish today, denying connections restarted Cajal. also removed all the memory DIMMs and reinstalled them. Cajal now recognizes 48 GB of RAM again (instead of just 24 GB). Project 2 moved … Continue reading →

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Wednesday 12/11/13

Posted on December 11, 2013 by admin

11:15a – 11:45p Ph Project Highly variable radius of gyration measurements for case with binding — suggests not equilbirated. Binding strength probably much to high. Up to .90, run again. Also increased density of K27me3 sites from 50% to 80%, … Continue reading →

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STORM2 BX-C 01/23/13

Posted on January 23, 2013 by admin

Setup Back-optics aligned Focus lock working Laser power: 647 laser at 200 mW source: 130 mW at power meter 405 laser: max power = 1.89 mW.  10% = .09 mW z-stage drift without focus lock is ~800 nm per min … Continue reading →

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Sunday 12/09/12

Posted on December 9, 2012 by admin

10:20 A – 7:30P, 9:30-11:00P STORM 750 1600 mA = 18 mW 1.45 mW 405 200 mW set 647 Made new Glox 750 not switching well.  Not sure if it’s just low labeling (405 looked okay but not strong). 647 … Continue reading →

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Monday 11/19/12

Posted on November 19, 2012 by admin

9:15 A — 7:15 P, 8:15P – 1:15 A Cell labeling hot washes for probe first slide (2x at 60C) 1x at RT remove and save primary antibodies at RT for primary first slide. PBT rinses (3x soln change, 3rd … Continue reading →

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STORM2 rebuild SOP

Posted on November 19, 2012 by admin

  Follow text file SOP for 750 laser. Aligning back optics place microscope in epi mode (12.000) Course tuning remove 20x, 100x objectives. screw in target objective in 100x objective position. remove final lens before scope body, add iris in … Continue reading →

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