Tag Archives: antibody labeling

Thursday 11/29/12

Posted on November 29, 2012 by admin

9:20 A — 7:15P, 8:15P – 10:00P Polytene cell Check polytene cell staining.  Faint glow of nuclear laminin visible.  pretty dim, does not have ring shape, see top and bottom (would be easier to distinguish as diffinitive stain by confocal). … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Thursday 11/29/12

Saturday 11/17/12

Posted on November 17, 2012 by admin

10:00 A – 12:00P, 3:30P – 8:00P, 9:00P-11:20P S2 Cell cent + chromatin/PcG Hot washes PBT washes block 4 hrs antibodies: H3K9me2, Pc, PCNA (Fisher) (1:3 across the top) rinse, block, secondaries O/N at 4C Harvard wins Yale Game Chromatic … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Saturday 11/17/12

Wednesday 10/17/12

Posted on October 17, 2012 by admin

9:10 A – 6:00P, 9:30P – 11:20 P start thawing DNA Fix Mix at 37C email BIRS team about NIH funding option update Need to coat new coverslips.  Contacting olympus about #1.5 vs #1 coverslips.  (Zeiss recommends only 1.5, strong … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Wednesday 10/17/12