9:45 A – 7:15P, 8:30P – 10:40 P
STORM
- Record z-calibration curve for STORM3
- Finish O/N run of Fab7 on STORM3
- Finish O/N run of BX-C embryos on STORM2. Ideal focus plane not matched between regions (this can be custom specified / modified in the xml file produced by Dave. Should try to fix focus issues next time).
Sort data-copying
- Delete _inv from Alistair7, now on Alistair5.
- Alistair5 and Alistair6 now completely full. All active projects on Alistair7 or 8.
- Move 2013-01-24_BXC from Alistair8 to Alistair7
- Copy data from STORM2 to Alistair7
Embryo labeling
- BX-C in situ (round 2) day 2: light hot wash 55C, 15 min
- Now in 4C in SSCT awaiting imaging.
- Embryo protein label check staining. H3K27me3 looks good.
Cell labeling
- mounted En-locus DAPI, 500 kbp DAPI and AATAT control DAPI slides
- mounted 500 kbp / DAPI / H3K27me3-750-Abcam / Cy3B-Dm0 labeled cells.
Fly Work
- et up MAT crosses for all remaining shRNA lines.
- Flip shRNA lines
- water rescue crosses
Writing
- shadow model for Stas
* # Rewrite draft of review in more Biophys J appropriate format.