12:00 P – 9:30 P
- 8:30-12P volleyball practice
- update probe & plasmid library
- update antibody labels
- collect virgins
- set up shRNA crosses x Pc shRNA2 and Psc shRNA 1 in collection cages
- PCR for in vitro transcription rxn to make new RNA probes
- Flip fly stocks
- Gel extracting PCR: AbdB-1A, AbdB-2B, AbdB-3B, Dfd-2A, Fab8A, 8EA, 7EB, Taf2, Tub1, + previous PCR cloning for iab4.6, 3.2 and Fab7
- All these 2 kb bands run very close — difficult to gel extract without a bit of overlap.
- new cage Pc-GFP
- staining cryo-sectioned embryos: