11:00A -5:30P
- Talk title for Poland
- wash out primary antibodies
- Nanodrop gel-extraction samples
- STORM cryo-section samples:
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- considerable out of focus light makes for hazy looking conventional images, though nuclei and membranes are clearly distinguishable in the respective channels.
- 647 STORM has good single molecule detection. However, out-of focus light in 488 and 750 channels washes out weaker single molecule emissions. No TIRF angle choice
- provides necessary contrast.
- submitted BIRS proposal