10:00 A – 11:45 P
- Check colonies from Bx-C round 1 cloning: all plates look good, no lawns.
- Gel-extract linearized Taf, Tub
- Gel-extract newly cloned Bx-C probes
- Antibody synthesis: antibodies 61-69
- Flip collection cages: 10:15 A
- Washing out primary antibody stain from Oligo-STORM rnd 5 embryos
- N-STORM training (see post)
- Ligate Bx-C probes
- Probe synthesis for AbdA, Antp, Ubx, tou, Taf and Tub,-dig
- incubating Oligo-STORM in secondary antibodies
- Finish probe synthesis for en, inv, and E(Pc)-dig
- pick colonies for Bx-C cloning.
- cut sections Pc 750 + H3K27-647 + Dm01.
- Order centromere AATAT 5x rpt Cy5 conjugated to develop DNA-FISH protocol.
- O/N fly plates -> new bottles.
- Flip Gal4-bcd3’UTR bottles.
- Oligo-STORM rnd 5 sample post-fixed, in PBT at 4C O/N.