11:00 A – 6:45P, 8:00P -12:00A
- flip fly collection cages, 11A
- Miniprep cultured colonies: 8E, 7E, 2E, AbdB2 and Fab8 (2 colonies each). Send for sequencing.
- Rearrange ultratome room, suspend on air-table, clean room.
- Flip collection cages, 3:15 A
- Analyzing run2 PC-GFP data (insight files look worse — dot’s much dimmer, TIRF angle probably insufficient. we’ll see how they compare).
- Analyzing Bcd hbyi data
- Moving Pc-750 data to Alistair2
- Analyzing Pc-750 K27-647 data
- Fix embryos: 5:00 P
- in situs day 1 to test new probes: taf, tub, Antp, AbdA, Ubx, en, inv, tou, E(pc), + sna as control.
- Cut sections of H4 (H3K27me3, H3K4me3)
- Clear Gal4-bcd3’utr cages, collect phenotypic virgins
- confocal analysis of all secondary controls. only very weak staining observed under 50%+ laser power and high gain. No ectopic nuclear bodies. Maybe the 4C secondary incubation doesn’t get that strange background dots.