9:45 A – 8:15P,
- Steven group meeting presentation
- sequencing results:
- AbdB-1A: sense strand. Use SP6 for antisense probe
- AbdB-1B: sense strand. Use SP6
- AbdB-3A: sense strand. poor quality read
- AbdB-3B: antisense strand. Use T7
- Dfd-2A: antisense strand: T7
- Dfd-2B: antisense strand: T7
- 5E-A: plus/minus. sense strand for noncoding msa sequence. Sp6
- 5E-B: plus/plus. antisense strand for noncoding msa: T7
- My group meeting presentation for Francis lab
- Scm homology to Pho, similar functional effect on knockdown — less Scm => PRC1/2 but not vice versa. Tethered Scm can drive mini-white silencing (unlike Pho), in a PRC1 dependent fashion.
- Paro has bio-tagged Pc that reportedly rescues embryonic lethal.
- Pc-GFP TIRF angle no good for 750 in run2 — all molecules are too dim.
- Picked colonies from latest cloning: 8E, 7E, 2E, Fab8, AbdB2.
- Running Pc-GFP run1 analysis through list2tif3d_color_drift_corrected with and without a 400 nm window z-filter.