10:30 A – 7:30P, 8:3oP – 10:40P
- check O/N confocal run, 13 embryos 2 channels 70 sections on speed 5 started at 8P, 12/13 finished. (several embryos were not substantially flattened, hence the extra sections).
- expansion of lens has changed focal position, many embryos not getting top layer of mRNA. Restarted run for embryos 2-12, speed 6, recalibrated z.
- Etching S7 AbdA AbdB coverslip 1 (good ribbon). Using new sodium ethoxide from freezer stock. First 15 min etch, resin sections clearly visible.
- second 15 min etch, resin sections clearly visible. Return for another 32 min etch (I think this is going to be a long one).
- Working on configuring 3d_daostorm scripts to run on odessey. Compiled all c-programs except for grid.c. README does not have directions on compiling grid.c, and: “gcc grid.c -o grid -lm” doesn’t work nor does “gcc grid.c -0 grid”
- calling sample script also gives an error on import library.datareader, even though library is definetely added to system path.
- No cylindrical lens on STORM4.
- STORM4 also not tiling correctly — images all flipped in steve (probably in hal too).
- Replaced rod structure with Graham and re-aligned camera using micron ruler slide. Minor further adjustments using bead slides. Field is moderately flat. fix time 3.5 hrs. Starting bead calibration movies, 5:15pm.
- Replacement seems to have fixed tiling. STORM imaging of AbdA. very little AbdB detectable.
- Confocal rnd 2 of mp07 (minus primary) embryos finished. Image 30C Mp07s?