Thursday 02/07/13

9:30 AM – 8:30P

STORM

• Finish O/N STORM imaging
• Pc-488 not bleaching well. Maybe 750 will be better.

Fly Work

• Esc virgins emerging — collecting. some non-Sp (Df/esc[2] — it’s okay). No males yet to score phenotype
• AM collection 2x mat alpha tub virgins * SCM shRNA line ready for embryo collection cages!
• rescore recombinant crosses with more embryos. 1 possible hit to recross!
• Cleared sim[D] and espl[D] bottles for virgin collection for confirmatory checks. Should also make espl,sim/TM6b stable lines with the 3 candidate stocks.
• Moved SCM shRNA F1s to collection cage
• PM virgin collections. No males yet for Esc mutants

MS review

• Finish requested review
• review my review
• submit to journal website

Coding

• strip simulated annealing from STORMfinderBeta zcalibration (use new wx-wy crossover leveling). see post.
• fxn_rebuild_mosaic crashes Monet. Should rewrite this to do the assembly more intelligently.
• seeing if I can get the default function call of figure zoom to display in the editor, where I can build off it, instead of just overload from scratch.
• — The zoom buttons toggle zoom mode on and off e.g. zoom on. Can also get current axis xmin and ymin. We’ll see how this goes.
• Wrote MosaicViewer.m, a stripped down version of fxn_rebuild_mosaic.m that uses a lot less memory.