Saturday 01/21/12

8:45 A — 2:00 P

  • in situs moved through resin, dense collection of embryos
  • 10 embedding capsules of embryos made for each of the 4 in situ tubes (+4 of tube 1 by KE for practice).
  • in situ embryos baking at 70C f0r 8hrs
  • test confocal images of new Drosophila lamin antibodies and new histone antibodies.
  • All histone antibodies look good, though H3 and H3K4 appear to have been switched on labels.
  • Dm01 (A84) looks definetely best and brightest.  antibodies from undiluted serum show no detectable staining.
  • Dm01-A84_H3K4me3_2

    Dm01-A84_H3K4me3_2

    Dm03-A195_H3K27me3

    Dm03-A195_H3K27me3

    Dm01-A84_H3K4me3

    Dm01-A84_H3K4me3

    WGA_Dm05-46_H3ac

    WGA_Dm05-46_H3ac

    Dm02-ADL67_H3

    Dm02-ADL67_H3

    WGA_H3ac

    WGA_H3ac

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