11:00A – 7:30P, 8:30P-11:30P
- Flip collection cages 11:20A
- Order Primers for Taf1, aTub84B, m13F/R
- Confocal: STORM prepped slides. Both 1hr and 35 min samples are substantially over etched, most of sample is gone. Try 20 min next (?).
- Confocal: MP08 embryos. No good rescue embryos on 1/17/12 slide.
- Attempted concentrating H3K27me3-647 antibody and ac-750 antibody using vivaspin. only 2 fold concentration increase for 750, though estimate now 2:1 405:750 instead of 18:8 (dye peaks dropped, antibody peak did not improve).
- Adapted dax2png code for more general application. Projected 750-dye check images (into data folder on ALISTAIR2).
- Discussion with Yari on confocal imaging of plasticized embryos. Should be able to see 647 in plastic. Trim face of plastic block. Put plastic block back in a cut embedding capsule (for support) and place on coverglass with a water drop or oil drop between glass and plastic (oil permeates the block somewhat and reduces stiffness of first sections).
- Working on snail paper text