Tuesday 01/31/12

10:00 A — 12:55 A

  • Flip fly cages (MP08 and yw)
  • Review JCC app.
  • Submit JCC app.
  • Working on snail paper final results/model section.
  • Syncing datafiles on snail patterning
  • sectioning: Cut 2 slides of s04(13-introns), one of slide 1, one of slide 3.  With KE.
  • etching slide 4 (13 introns +H3K4 and ftz).  20 min — etching not complete.  Restart.
  • Ran etching too long, 1 hour total.  (should set timer!)
  • Over-etching might be destroying the H3K4-647 label before the section is completely efaced?  low signal.
  • STORM imaging of Slide 45.  Most sections destroyed by etching.
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