10:00 -8:00P, 9:00P -11:20P
- Flip fly cages
- Finish clean up O/N STORM run. Coopying files from Alistair5 to Alistair4.
- move in situ probe-test slides into boxes. (Move archive boxes into 4C bin to make more space in 4C drawer?)
- 11:00 A – 3:00 P confocal test new probe stains: all probes work!
- tub pretty highly expressed, substantial cytoplasmic mRNA from exon. Can still ID nascent dots. Taf not well expressed.
- antibody labeling with KE: new donkey anti-mouse and donkey anti-rabbit (from R&D Systems, not Jackson). 1 sample new Jackson antibody. Labeling reaction less efficient than previous with Jackson, upped concentration
- Fix embryos, 1-6 hrs.
- Etch Pc-GFP. First round forgot to add beads.
- First Pc-GFP etched 1 hr 25 min, had some wrinkles. Difficulty locating sample, may be over etched?
- Forgot to shoot z-calibration on beads this last-night this morning. 3D bead fields don’t replace z-calibration movie of programmed steps.
- Start test stains of new PCNA antibodies and newly labeled