Thursday 07/10/14

10:00 am – 7:30 pm, 11:20 pm – 12:10 am

Post-doc candidate visit

  • see notes

Chromatin project figure making

internal domains

  • need examples for figures

Multi-color region analysis

  • Black:Yellow
    • F01 :: F02
    • F07 :: F06
    • F11 :: F12
    • G08 :: G06
  • Blue :: Black
    • G08 :: G09
  • Blue :: Yellow
    • F03 :: F02
    • F05 :: F06
    • G01 :: F12
    • G05 :: G06
  • Blue :: Blue
    • G01 :: G02
    • G02 :: G03
    • F03 :: F04
    • F04 :: F05
  • Yellow :: Yellow
    • L3E06 :: L3E07
    • L3E07 :: L3E08 (?)
  • Black :: Black
    • L3D11 :: L3D12

Analysis methods

  • want an Rg analog for overlap — we don’t want differences in the fringes of scattered particles to make a difference in the ‘volume’ fraction overlap.
  • to be Rg analog, metric should treat localizations as density
  • We could also make this independent of binning — which was an annoying free parameter last time.
  • Proposal:
    • fraction of localizations in one channel with at least N localization within distance D (relative to total localizations making up domain).

F6F5separate2

F6F5separate

F6F5embrace

F6F5overlap

NeedDensityCorrectedOverlap

RandomWalkPolymerInternal

RandomWalkOverlap

F6F5separate3

New simulations

  • Free polymer with spherical confinement to a default density of 0.3 particles per cubic nanometer (if bond size is 1 nm).
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