10:30 A – 7:30P,
Data analysis
- Analyzing Hp1a centromere data
- Labeling density still somewhat low, centromere certainly not ‘coated’ with Hp1a by imaging.
- Might be an antibody accessability thing. Should compare with bio-labeled centromere probe. I remember when I last did bio-labeled centromere probe in embryos the labeling density seemed much less than I typically observe with directly labeled probe. Should also do a test with HP1a in 647.
Working on lab meeting presentation layout coverglass Ucryo section
- coverslip slid over too the rest on top of the non-silanated labeling area of the coverslip (bringing the solution with it at least), leaving coverglass dry).
- Sections still look well labeled on Turnkey
- store at 4C for STORM imaging time tomorrow
Literature
- Eskland 2010 shows decompaction of hox loci in ES cells upon RA induction. Decompaction is also observed in non-induced ES cells upon PRC1 (Ring1B) and PRC2 (Eed) knockdown. PRC2