10:00a – 11:30p
Meetings
- lab meeting (post-doc interview). See notes
- post-doc chat
- fixing up slides for post-doc chats, moving onto google docs so that the presentation is mobile
- google docs having issues loading images in presentations that used to display just fine.
- a number of images display properly when run in presentation mode. some still fail to load.
- libreOffice keeps freezing when I try to update my current set of slides. what is this?
- attempting to download. This actually works and displays figures correctly
- notes for next time: fewer stories, more background.
- post-doc dinner
Chromatin imaging (single color)
- Finish O/N STORM of F03 spots on STORM4.
- Set up new O/N STORM of F04 spots on STORM4
- test out Hal upgrades on STORM4?
cell culture
- Set up new 12-well plate of Kc167 cells for fixation and hybe prep
- passage cells (new media to both active flasks).
- fixing cells (settled out perfectly confluent).
New stains
- G4-P1-A647
- F5-P1-A647
- F3 + F4 + F5 -P1-A647
- F5-P1-A647 + F6-P3-A750
Ph project
Modeling
- reasonable results for PhM effects in capping model
- need to re-run for diff binding model (now in process)
Data analysis
- start processing cells through STORMrender (used to parse out individual cells) for Ph analysis.
- over-expression I think is killing the structure. The lower expressing wt look much better.