Tag Archives: embedding

Monday 10/15/12

Posted on October 15, 2012 by admin

9:30 A – 7:30 P Flip collection plate on MTD flies.  starting to lay…. sorta. looking at aptamers for histone labeling (see Mendeley). DNA-FISH + protein prep for STORM protocol test try 2:  samples now embedding. [spreadsheet 0AjSqkxgziU1YdG90a29UaU5ua1FLUXZ5LTFmM1pMSlE 602 302 sheet=8] … Continue reading →

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Thursday 07/5/12

Posted on July 5, 2012 by admin

9:50A – 7:15P, 9:00P – 10:45P check nascent transcripts of AbdA (red) and Antp (green) on yw and Pc O/N short-fix embryos.  No obvious staining on turnkey. Should check 6/11 embryos as positive controls and 6/25 embryos to see effect … Continue reading →

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Thursday 04/05/12

Posted on April 5, 2012 by admin

10:00 A — 8:30 P Take GFP embryos out of 4C, incubating at RT heating up 2x SSCT for hot wash mounted oligo-paint embryos before and after washes (with WGA-488 for post-wash embryos).  Remaining embryos at 4C Cut sections of … Continue reading →

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Saturday 03/24/12

Posted on March 24, 2012 by admin

10:15 A Assemble draft protocol of embryo multiplex RNA-Protein STORM send to Stacie at Stowers. 5 mL of culture in probably too much to get good aeration in small round-bottom tubes.  Try 2 mL next time.  use Zhuang shaker, it … Continue reading →

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Monday 11/19/11

Posted on December 19, 2011 by admin

10:50 A -7:05 test Invitrogen DNA dyes in whole-mount yw 2-4 hr embryos. embedding engrailed and hox labeled embryos, in 70% resin: 11:30A washing out WGA labeling in bcd/ftz/K4 embryos. test Draq5 on sections: also does not label etching new … Continue reading →

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