10:50 A -7:05
- test Invitrogen DNA dyes in whole-mount yw 2-4 hr embryos.
- embedding engrailed and hox labeled embryos, in 70% resin: 11:30A
- washing out WGA labeling in bcd/ftz/K4 embryos.
- test Draq5 on sections: also does not label
- etching new slide in case DNA degradation is a problem for testing labeling (Hoechst should have worked better?)
- Even in newly etched sections, Draq5 and Hoescht don’t label. Maybe long etching removes DNA? No convincing intronic mRNA stains yet in sectioned embryos.
- post-fix WGA-labeled bcd/ftz/K4 embryos.
- Add draq5 to K4 labeled embryos.
- Invitrogen DNA dyes label membranes very strongly, makes finding sample on confocal many-many times easier. Also shows that post-cutting labeling does work. Trying now with cy3B to have some a membrane dye with better photo-switching.
- Etching long strips of MP09 sectioned embryos (slide 7 from in situs on 12/9). Look for any sign of 647 labeled mRNA in any of the sections.