Tuesday 12/20/11

10:50 A – 8:30P

  • remove engrailed and hox stained and embedded embryos from 70C oven
  • continue embedding of bcd/ftz/K4 protein/RNA multiplex stains — onto 50:50 resin:EtOH
  • Cut sections: en-flu and AbdA, at both 100 nm and 50 nm.  (7hrs).  good ribbons, need a way to add water back to bath to compensate for evaporation.  Increasing humidifier should help.  Humidifier out of water = sections don’t stick together at all, zero.
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