Wednesday 12-21-11

7:40 A – 11:00P

  • breakdown O/N confocal of MP08
  • write script to extract nuclei from cytoplasmic localized mRNAs (sog and snail).  Extract nuclei for s05_MP08_b.
  • first pass dot counting for s05_MP08_b and for snaD_b.  Data not analyzed yet.
  • cutting samples on ultratome: Antp, AbdA and bcd.
  • STORM imaging of s07_MP09. Trying to get 3D superresolution structure of broad transcriptional foci.  Can locate foci in consecutive 100 nm sections.  difficulty imaging.  Attempting O/N run started ~11P.
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