9:45 A — 11:00P
- Washing out O/N 4C antibodies from new protein stains
- Flip Fly stocks
- Flip vials and enter new GFP lines into fly stocks.
- Start expanding Gal4 bcd-3′ UTR (to try crossing into PcG RNAi lines. will need lots of virgin females for this line to work).
- Cut 2 slides of a couple sections of Dm01 multi-labeled embryos.
- Day 2 of protein stains.
- confocal check of new Pc and Hb Bcd stains.
- Hb Bcd slide has same apparently PcG bodies as Bcd slide (not surprising since treated side by side). Need to repeat. Hb weak but detectable.
- working on STORM analysis code: automated scripts from matlab (in place of Python scripts)