9:50 A – 12:10 A
- Transfer PcG and Hb/Bcd samples to ethanol for embedding
- Running Insight3 analysis on single section images from Tuesday / Wednesday STORM session.
- Discussion with Graham and Colenso: Try switching to BME for
- Drosophila PcG shRNA lines arrived. Need maternal driver still. Need to start stock list.
- Order new TF antibodies from Hybridoma Bank:
- 4D9 anti-engrailed/invected; 3C10 anti-even skipped, ESC 53.1; 6E8 (PSC); anti-Scr 6H4.1; anti-ABD-B (1A2E9); Ubx FP3.38; C1A9 (HP1);
- Fly lines arrived: PcG shRNA knockdown
- Discuss with Ben maternal drivers. Recommends contact Perrimon for master maternal Gal4 line (see email).
- Smoothing out code for automated image projection.
- Exporting super-resolution figures of Psc on chromatin for fly meeting talk.
- wrote new automated code for for running Insight from matlab. Works on Monet and Tuck.
- First pass of the 3D H2 data not look good.
- Attempting 2D analysis of 3D H2 data for comparison.
- Resin embedding of samples. Samples in oven at 6P.