10:10 A — 11:20 P
- move samples from 4C, change 100% EtOH
- STORM: setup 10x objective for finding samples. substantial offset, need to calibrate.
- Need to set laser powers to zero but have lasers on before starting dave!
- Ordered Bloomington GFP stocks:
-
9593 w[1118]; P{w[+mC]=Pc[T:Avic\GFP-EGFP]}3 29018 P{w[+mC]=EGFP-bcd}1, y[1] w[1118]; th[1] st[1] kni[ri-1] bcd[6] rn[roe-1] p[p] 30870 y[1] w[*]; PBac{y[+mDint2] w[+mC]=en-EGFP.S}VK00033 30871 y[1] w[*]; PBac{y[+mDint2] w[+mC]=eve-EGFP.S}VK00033 30872 y[1] w[*]; PBac{y[+mDint2] w[+mC]=slp2-EGFP.S}VK00033/TM3, Sb[1] 30874 y[1] w[*]; PBac{y[+mDint2] w[+mC]=tll-EGFP.S}VK00037 31419 w[*]; P{w[+mC]=UASp-bnb[N20].mEos}1 - continuing embryo staining, day 4: embedding.
- Flip fly cage 1P. (weak egg lay).
- STORM: slide H3, 750 might be okay.
- STORM: slide S2, running O/N.
- Confocal imaging (see summary posts).
- Embedding embryos en mass in cut, circular embedding cases.