slide H1: 4 color anti-nuclear lamin test
slide H2: Directly labeled antibodies: Dm01-488(green) H3K27me3-Cy3B (red), H3-Cy5 (blue), H3K27ac-750 (not shown)
slide H4: H3K27me3-Cy3B (red) and H3K4me3-Cy5 (blue) along with 405 background (green).
slide H4: earlier embryo: H3K27me3 ‘clusters?’ (red) over H3K4me3 diffuse staining (slide H4)
Slide H3: H3K27ac-488 (green, failed), 27ac-Cy5 (blue), Dm0 (red)
405-K27me3, 405,647-K27ac, Cy3B-Dm0, late cc14 embryo.