9:50 A – 1:00A
- Remove and keep primaries from all staining reactions (except traditional snail labeling of MP08 embryos)
- Washing embryos, in situs / staining reactions day 3.
- new stock of 1x PBT
- clean frozen fly cages
- Flip fly cage: 10:30 A
- Very weak egg lay by 1:30P, no embryos fixed.
- doubled concentration of secondaries from previous reactions (used at 1:100). Typical homemade secondaries are at 0.3mg/mL, commerical secondaries (used at 1:500) are 2 mg/mL, so should probably use at 1:75.
- looking up fly lines with targeted insertion sites to incorporate GFPs into PcGs.
- # Order Pc-GFP (#9593)
- # 31190 y1 v1; P{TRiP.JF01705}attP2
- mounted samples
- confocal