10:00 A – 8:45P
- in situs day 2 (no KE)
- antibody test try 2: 15 min fix <24 hr methanol treated embryos stained for PcG proteins.
- much higher concentration of histone primary antibodies test stain (record)
- Trial run of new protocol: acetic acid fix embryos: fix phase works fine. Embryos still float in heptane methanol mix.
- need to rinse repeatedly in methanol before moving to PBT, or residue heptane and tween react creating a opaque sticky colloid with floating embryos. Embryos will sink in clean methanol.
- embryos failed to devitilinize on first methanol heptane shake.
- after cleaning methanol, resuspended in heptane and vortex until embryos sink. hopefully now embryos are properly devitilinized.
- Reorganizing freezers: probe plasmids, archived embryos, and aliquots of salmon sperm and heparin have been moved to -20C in the basement.
- Next up: separate probes into frequently used and rest of the library and move the library down to the basement.
- Flip MP09 flies — flies lay really well, tons of larvae. pupation not as successful.
- Froze large cages of MPo8 and yw flies.
- New cage of yw flies started. Will need to make more plates soon.
- Fixed issue with batchinsightm — parameter files seem to be lacking (save options from insight3).
- Running analysis of Pc2 STORM data with low threshold (can always screen later image by photon count, so let’s get all possible ones processed to start with).
- batchinisight still gives errors for ubx dataset.